Histone H4ac (pan-acetyl) antibody (mAb) (Clone 3HH4-2C2)
Host / Isotype
Mouse / IgG1, k
Reactivity
Human, Wide Range Predicted
Applications
ICC, IF, IHC, WB
CloneNo.
3HH4-2C2
Cat No : 39967 39967
Synonyms
Validation Data Gallery
Product Information
| Tested Applications | ICC, IF, IHC, WB |
| Tested Reactivity | Human, Wide Range Predicted |
| Host / Isotype | Mouse / IgG1, k |
| Class | Monoclonal |
| Type | Antibody |
| Modification | Acetylated |
| Immunogen | This Histone H4 acetyl antibody was raised against a peptide corresponding to amino acids 1- 24 of human histone H4 acetylated at lysines 5, 8, 12 and 16. Due to sequence similarity, cross-reactivity has been observed with acetylated Histone H2A. |
| Full Name | Histone H4ac (pan-acetyl) antibody (mAb) (Clone 3HH4-2C2) |
| Synonyms | histone H4, histone, H4, histone-H4, histoneH4, mAb, monoclonal, antibody, antibodies, 3HH4-2C2 |
| Molecular weight | 8 kDa |
| GenBank accession number | NP_778224 |
| RRID | AB_2793413 |
| Purification Method | Protein A Chromatography |
| Buffer | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H4 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as Hat1 or Gcn5. Acetylation of histones is often associated with transcriptional activation.