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Histone H4H18ph antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Wide Range Predicted

Applications

DB, WB

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Cat No : 61413,61414 61413

Synonyms

Histone H4, H4H18ph, histone, H4, histidine, phospho, phosphorylation, antibody, polyclonal, pAb, wb, db, western blotting, dot blotting, sample

Validation Data Gallery

  • Dot blot of Histone H4 phospho Histidine 18 antibody (pAb). Dot blot analysis was used to confirm the specificity of H4H18ph antibody for phosphorylation on Histidine 18 of histone H4. Peptides corresponding to the immunogen and an unmodified sequence were spotted onto PVDF and probed with H4H18ph at a 1:1,000 dilution. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: unmodified peptide. Lane 2: H4H18ph peptide.

    Dot blot of Histone H4 phospho Histidine 18 antibody (pAb). Dot blot analysis was used to confirm the specificity of H4H18ph antibody for phosphorylation on Histidine 18 of histone H4. Peptides corresponding to the immunogen and an unmodified sequence were spotted onto PVDF and probed with H4H18ph at a 1:1,000 dilution. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: unmodified peptide. Lane 2: H4H18ph peptide.

Product Information

Tested Applications DB, WB

Tested Reactivity Human, Wide Range Predicted
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
ModificationPhosphorylated
Immunogen This antibody was raised against a synthetic peptide containing a 3-phosphohistidine analog at Histidine 18 of human Histone H4.
Full Name Histone H4H18ph antibody (pAb)
Synonyms Histone H4, H4H18ph, histone, H4, histidine, phospho, phosphorylation, antibody, polyclonal, pAb, wb, db, western blotting, dot blotting, sample
Molecular weight 8 kDa
GenBank accession numberNP_778224
RRIDAB_2793626
Purification Method Protein A Chromatography
Buffer Purified IgG containing 30% glycerol and 0.035% sodium azide. This serum was depleted over unmodified peptide before protein A purification. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Phosphorylation of histidines in Histone H4 has been known to occur in liver cells and may be associated with proliferation of hepatocytes during regeneration of the liver following damage.