Histone H4 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Wide Range Predicted

Applications

ChIP, ChIP-Seq, WB

Cat No : 61299,61300

Synonyms

Histone H4, H4, Histone, variant, chromatin, nucleosome, antibody, antibodies, polyclonal, chromatin immunoprecipitation, chip, sample, pAb, western blotting

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Validation Data Gallery

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  Histone H4 antibody (pAb) tested by ChIP Performed using HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) and 10 ul of Histone H4 antibody or 10 ul of rabbit IgG as a negative control. Real time, quantitative PCR was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the PABPC1 (+ 0.5) gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG (ddCT method).

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  Histone H4 antibody (pAb) tested by Western blot Nuclear extract of HeLa cells (30 ug) probed with Histone H4 antibody (1:1,000).

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Product Information

• Tested Applications: ChIP, ChIP-Seq, WB

  Applications Validated by Active Motif: ChIP: 2 - 10 ul per ChIP WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. For Histone H4, we also offer AbFlex Histone H4 Recombinant Antibody (rAb). For details, see Catalog No. 91295.

• Tested Reactivity: Human, Wide Range Predicted
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: This Histone H4 antibody was raised against a peptide corresponding to amino acids 43- 54 of human histone H4.
• Full Name: Histone H4 antibody (pAb)
• Synonyms: Histone H4, H4, Histone, variant, chromatin, nucleosome, antibody, antibodies, polyclonal, chromatin immunoprecipitation, chip, sample, pAb, western blotting
• Molecular weight: 8 kDa
• GenBank accession number: NP_778224
• RRID: AB_2650524
• Purification Method: Affinity Purified
• Buffer: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Histone H4 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.