Histone H4 antibody (mAb) (Clone MABI 0400)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Wide Range Predicted

Applications

ChIP, WB

CloneNo.

MABI 0400

Cat No : 61521

Synonyms

Histone H4, H4, Histone, variant, chromatin, nucleosome, antibody, antibodies, monoclonal, mab, mabi, chromatin immunoprecipitation, chip, western blotting, wb

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Validation Data Gallery

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  Histone H4 antibody (mAb) tested by ChIP analysis. ChIP performed using the ChIP-IT Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 3 ug of Histone H4 (mAb) or the equivalent amount of mouse IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the PABPC1 gene. Data are presented as Fold Enrichment of antibody versus control IgG (ddCT method).

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  Histone H4 antibody (mAb) tested by Western blot. Nuclear extract (20 ug) of HeLa cells probed with the Histone H4 antibody at 2 ug/ml.

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Product Information

• Tested Applications: ChIP, WB

  Applications Validated by Active Motif: ChIP: 2 - 5 ug per ChIP WB*: 0.5 - 2 ug/ml dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For Histone H4, we also offer AbFlex Histone H4 Recombinant Antibody (rAb). For details, see Catalog No. 91295.

• Tested Reactivity: Human, Wide Range Predicted
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: This antibody was raised against a synthetic peptide containing human Histone H4.
• Full Name: Histone H4 antibody (mAb) (Clone MABI 0400)
• Synonyms: Histone H4, H4, Histone, variant, chromatin, nucleosome, antibody, antibodies, monoclonal, mab, mabi, chromatin immunoprecipitation, chip, western blotting, wb
• Molecular weight: 8 kDa
• GenBank accession number: NP_778224
• RRID: AB_2793667
• Purification Method: Protein G Chromatography
• Buffer: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression.