Histone H3K4me3 antibody (pAb)
Host / Isotype
Rabbit / IgG
Reactivity
Budding Yeast, Human, Wide Range Predicted
Applications
ChIP, ChIP-Seq, DB, ICC, IF, WB
Cat No : 39016,39915,39916 39915
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
ChIP, ChIP-Seq, DB, ICC, IF, WB
Applications Validated by Active Motif: ChIP: 3 ug per ChIP ChIP-Seq: 4 ug each ICC/IF: 1 - 2 ug/ml dilution WB*: 2 - 4 ug/ml dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. |
| Tested Reactivity | Budding Yeast, Human, Wide Range Predicted |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Modification | Methylated |
| Immunogen | This Histone H3 trimethyl Lys4 antibody was raised against a peptide including trimethyl-lysine 4 of histone H3. |
| Full Name | Histone H3K4me3 antibody (pAb) |
| Synonyms | histone H3, histone, H3, histone-H3, histoneH3, pAb, polyclonal, H3K4me3, Histone H3 trimethyl Lys4, Histone H3 trimethyl Lysine 4, Histone H3 trimethyl Lys 4, Histone H3 trimethyl K4, Histone H3 trimethyl K 4, Histone H3 tri-methyl Lys4, Histone H3 tri-methyl Lysine 4, Histone H3 tri-methyl Lys 4, Histone H3 tri-methyl K4, Histone H3 tri-methyl K 4, Histone H3 3Me Lys4, Histone H3 3Me Lysine 4, Histone H3 3Me Lys 4, Histone H3 3Me K4, Histone H3 3Me K 4, Histone H3 Me3 Lys4, Histone H3 Me3 Lysine 4, Histone H3 Me3 Lys 4, Histone H3 Me3 K4, Histone H3 Me3 K 4, antibody, antibodies, H3K4Me3, sample, chiphsabs |
| Molecular weight | 17 kDa |
| GenBank accession number | NP_003522 |
| RRID | AB_2687512 |
| Purification Method | Protein A Chromatography |
| Buffer | Purified IgG in PBS (pH 7.5) with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version (Catalog No. 39159) of this antibody is also available. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.