GLTP Polyclonal antibody

• Featured Product
• KD/KO Validated

GLTP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 10850-1-AP

Synonyms

Glycolipid transfer protein

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Validation Data Gallery

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  WB analysis using 10850-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10850-1-AP (GLTP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10850-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10850-1-AP (GLTP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 10850-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10850-1-AP (GLTP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, mouse cerebellum tissue, rat brain tissue, HeLa cells, K-562 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 1 publications below

Product Information

10850-1-AP targets GLTP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLTP fusion protein Ag1293
• Full Name: glycolipid transfer protein
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC009932
• Gene Symbol: GLTP
• Gene ID (NCBI): 51228
• RRID: AB_2294627
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; miR-196a Upregulation Contributes to Gefitinib Resistance through Inhibiting GLTP Expression.; Authors - Bing-Jie Liu
mouse	WB	Arch Toxicol; Peroxisome proliferator-activated receptor α attenuates high-cholesterol diet-induced toxicity and pro-thrombotic effects in mice.; Authors - Yu Lu
mouse	WB	Hypertens Res; Effects of hypertension and antihypertensive treatments on sulfatide levels in serum and its metabolism.; Authors - Ran Guo
mouse	WB	Glycoconj J; Age-dependent PPARα activation induces hepatic sulfatide accumulation in transgenic mice carrying the hepatitis C virus core gene.; Authors - Yangyang Tian
human	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Upregulation of human glycolipid transfer protein (GLTP) induces necroptosis in colon carcinoma cells.; Authors - Shrawan Kumar Mishra
mouse	WB,IF	Mol Neurobiol; Role of Gltp in Maturation of Oligodendrocytes Under the Regulation of Nkx2.2; Authors - Chengfu Zhang