ERK5 Polyclonal antibody, PBS Only

ERK5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11164-1-PBS

Synonyms

ERK 5, MAPK7, MAPK 7, Extracellular signal-regulated kinase 5, ERK-5

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Validation Data Gallery

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  WB analysis of SKOV-3 using 11164-1-AP (same clone as 11164-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11164-1-AP (ERK5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11164-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 11164-1-AP (same clone as 11164-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11164-1-AP (ERK5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11164-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 11164-1-AP (same clone as 11164-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11164-1-AP (ERK5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11164-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 11164-1-AP (same clone as 11164-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11164-1-AP (ERK5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11164-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 11164-1-AP (same clone as 11164-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11164-1-AP (ERK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11164-1-PBS in a different storage buffer formulation.

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Product Information

11164-1-PBS targets ERK5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERK5 fusion protein Ag1648
• Full Name: mitogen-activated protein kinase 7
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC009963
• Gene Symbol: MAPK7
• Gene ID (NCBI): 5598
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.