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DOHH Polyclonal antibody, PBS Only

DOHH Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18226-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DOHH Polyclonal antibody (18226-1-AP)

    WB analysis using 18226-1-AP (same clone as 18226-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18226-1-AP (DOHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18226-1-PBS in a different storage buffer formulation.

Product Information

18226-1-PBS targets DOHH in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DOHH fusion protein Ag13072
Full Name deoxyhypusine hydroxylase/monooxygenase
Calculated Molecular Weight 33 kDa
Observed Molecular Weight30-35 kDa
GenBank Accession NumberBC002817
Gene Symbol DOHH
Gene ID (NCBI) 83475
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

eoxyhypusine hydroxylase (DOHH) is the enzyme catalyzing the second step in the post-translational synthesis of hypusine [Nε-(4-amino-2-hydroxybutyl)lysine] in the eukaryotic initiation factor 5A (eIF5A). DOHH has been reported to mediate several crucial cellular functions, including cellular proliferation, differentiation and apoptosis. Moreover, previous studies have established that DOHH is highly involved in several essential biological processes driving human diseases including cancer growth, malarial drug resistance, and HIV-1 replication. In particular, the DOHH/eIF5A signaling pathway was revealed to mediate the ability of nerve growth factor to enhance neuronal growth and survival, highlighting the importance of DOHH in neuroprotection(PMID: 35007708, PMID: 16371467, PMID: 22908221, PMID: 19706422).