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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-15 protein

Activity(Primary)

0.01-0.15 ng/mL in reporter assay

Specific Activity

minimally 3.50 x 107 IU/mg

Species Reactivity

human

Purity

>95%

Cat no : HZ-1337

Synonyms

Interleukin 15, IL15, IL-15MGC9721, Interleukin-15, IL-15

Validation Data Gallery

  • Purity of recombinant human IL-15 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-15 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-15 (Cat no: HZ-1323) stimulates dose-dependent proliferation of the NK-92 human natural killer cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. NK-92 cells were treated with increasing concentrations of recombinant IL-15 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.07-0.37 ng/mL.

    Recombinant human IL-15 (Cat no: HZ-1323) stimulates dose-dependent proliferation of the NK-92 human natural killer cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. NK-92 cells were treated with increasing concentrations of recombinant IL-15 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.07-0.37 ng/mL.

  • Recombinant human IL-15 (Cat no: HZ-1337) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 0.01-0.15 ng/mL EC50.

    Recombinant human IL-15 (Cat no: HZ-1337) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 0.01-0.15 ng/mL EC50.

Technical Specifications

GeneID 3600
Species Human
Expression HEK293
Activity(Primary) 0.01-0.15 ng/mL in reporter assay
Specific Activityminimally 3.50 x 107 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP40933
Molecular Mass 12 to 24 kDa reduced, 11 to 23 kDa non-reduced, monomer, glycosylated
Formulation 50 mM Acetate pH 4.0 + 150mM NaCl
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

IL-15 is a 4-α-helix bundle cytokine playing a pivotal role in stimulation of both innate and adaptive immune cells. It is produced primarily by keratinocytes, skeletal muscle cells, monocytes, and CD4+ T cells. It is a member of the common gamma chain family. The members of the common gamma chain family include the IL-2, IL-4, IL-7, IL-9, and IL-21 and require binding to the common γ chain receptor for activation. It can be used in growth and maintenance of T and NK cells. It is also shown to be used in proliferation and functional effect of T cells for adoptive cell therapy. It is a glycosylated protein and HumanKine IL-15 appear as 12.5-25 kDa bands (PMID: 24587813, 26627006, 27849617, 31250350)

Synonyms

Interleukin 15, IL15, IL-15MGC9721, Interleukin-15, IL-15