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LRRC2 Recombinant antibody

LRRC2 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241132G1

Cat no : 84404-1-RR

Synonyms

Leucine-rich repeat-containing protein 2, 241132G1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using LRRC2 Recombinant antibody (84404-1-RR)

    IHC staining of mouse skeletal muscle using 84404-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84404-1-RR (LRRC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using LRRC2 Recombinant antibody (84404-1-RR)

    IHC staining of mouse skeletal muscle using 84404-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84404-1-RR (LRRC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using LRRC2 Recombinant antibody (84404-1-RR)

    IF Staining of U-251 using 84404-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using LRRC2 antibody (84404-1-RR, Clone: 241132G1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84404-1-RR

    Affinity and Kinetic Characterization of 84404-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84404-1-RR against Human LRRC2 were performed. The affinity constant is 26.2 nM.

Tested Applications

Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84404-1-RR is LRRC2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LRRC2 fusion protein Ag27830
Full Name leucine rich repeat containing 2
Calculated Molecular Weight 371 aa, 43 kDa
GenBank Accession NumberBC029118
Gene Symbol LRRC2
Gene ID (NCBI) 79442
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Leucine Rich Repeat Containing 2 (LRRC2) was found to be localized to the mitochondria in human cells and transcriptionally regulated by the mitochondrial master regulator Pgc-1α. It has been reported that Lrrc2 transcript abundance correlates with that of β-MHC, a canonical marker of cardiac hypertrophy in humans, and experimentally demonstrated an elevation in Lrrc2 transcript in vitro and in vivo rodent models of cardiac hypertrophy as well as in patients with dilated cardiomyopathy. (PMID: 28158196)

Protocols

Product Specific Protocols
IHC protocol for LRRC2 antibody 84404-1-RRDownload protocol
IF protocol for LRRC2 antibody 84404-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols