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SEMA3B Recombinant antibody

SEMA3B Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241171A7

Cat no : 84080-3-RR

Synonyms

SEMA5, Sema V, Sema A(V), SemA, LUCA 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEMA3B Recombinant antibody (84080-3-RR)

    WB analysis using 84080-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84080-3-RR (SEMA3B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, U-251 cells, SK-N-SH cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84080-3-RR is SEMA3B Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SEMA3B fusion protein Ag25163
Full Name sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
Observed Molecular Weight83 kDa
GenBank Accession NumberBC009113
Gene Symbol SEMA3B
Gene ID (NCBI) 7869
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEMA3B is a class 3 semaphorin/collapsin family member containing 749 highly conserved, NH2 terminal, semaphorin amino acids. SEMA3B is characterized as a strong suppressing factor of the proliferation of cancerous cells and also by its anti-angiogenic effect. SEMA3B has been found in lung cancer, ovarian cancer, hepatocellular carcinoma, and cholangiocarcinoma. SEMA3B is expressed abundantly but differentially in various neural and nonneural tissues. The calculated molecular weight of SEMA3B is 83 kDa (PMID: 30914894, PMID: 32410560).

Protocols

Product Specific Protocols
WB protocol for SEMA3B antibody 84080-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols