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PARP14 Recombinant antibody

PARP14 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241078C11

Cat no : 84039-6-RR

Synonyms

PARP 14, KIAA1268, BAL2, 241078C11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PARP14 Recombinant antibody (84039-6-RR)

    WB analysis using 84039-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84039-6-RR (PARP14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using PARP14 Recombinant antibody (84039-6-RR)

    IHC staining of mouse colon using 84039-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84039-6-RR (PARP14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, U2OS cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84039-6-RR is PARP14 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PARP14 fusion protein Ag35007
Full Name poly (ADP-ribose) polymerase family, member 14
Calculated Molecular Weight203kd
Observed Molecular Weight200 kDa
GenBank Accession NumberNM_017554
Gene Symbol PARP14
Gene ID (NCBI) 54625
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARP14 (poly (ADP-ribose) polymerase family, member 14) is a PARP family member that has roles in DNA repair, B cell regulation, and focal adhesion (PMID: 25753673). The largest of all the human PARPs is PARP14. PARP14 has been reported to regulate several different pathways involved in immunity, inflammation, and genome stability (PMID: 37703374).

Protocols

Product Specific Protocols
WB protocol for PARP14 antibody 84039-6-RRDownload protocol
IHC protocol for PARP14 antibody 84039-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols