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CLEC12A Recombinant antibody

CLEC12A Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240791C2

Cat no : 83680-5-RR

Synonyms

CLL-1, CLL1, CLL 1, CD371, C type lectin like molecule 1

Validation Data Gallery

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  • Western Blot (WB) analysis of U-937 cells using CLEC12A Recombinant antibody (83680-5-RR)

    WB analysis of U-937 using 83680-5-RR

    U-937 cells were subjected to SDS PAGE followed by western blot with 83680-5-RR (CLEC12A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83680-5-RR

    Affinity and Kinetic Characterization of 83680-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83680-5-RR against Human CLEC12A were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inU-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83680-5-RR is CLEC12A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name C-type lectin domain family 12, member A
Calculated Molecular Weight31 kDa
Observed Molecular Weight55-75 kDa
GenBank Accession NumberBC126291
Gene Symbol CLEC12A
Gene ID (NCBI) 160364
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLEC12A, also known as CD371, MICL, DCAL-2 or CLL-1, is a type II transmembrane glycoprotein and a member of the C-type lectin superfamily (PMID: 30401586). This protein, which contains a single C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), is related to LOX-1 and Dectin-1 and is variably spliced and highly N-glycosylated (PMID: 14739280). Its ITIM sequence recruits the tyrosine phosphatases SHP-1 and SHP-2 to negatively regulate Syk signaling. In humans, CLEC12A is mainly expressed on myeloid cells, being found on monocytes, granulocytes, macrophages and DC subsets, and at lower levels on CD4+ T cells (PMID: 32477350). The observed molecular weight of CLEC12A is between 40 and 75 kDa, which is much higher than the predicted molecular weight due to glycosylation (PMID: 14739280).

Protocols

Product Specific Protocols
WB protocol for CLEC12A antibody 83680-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols