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MPHOSPH9 Recombinant antibody

MPHOSPH9 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240182D6

Cat no : 83397-5-RR

Synonyms

MPP9, MPP 9, M-phase phosphoprotein 9, 240182D6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MPHOSPH9 Recombinant antibody (83397-5-RR)

    WB analysis using 83397-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83397-5-RR (MPHOSPH9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MPHOSPH9 Recombinant antibody (83397-5-RR)

    WB analysis using 83397-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83397-5-RR (MPHOSPH9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using MPHOSPH9 Recombinant antibody (83397-5-RR)

    FC experiment of A431 using 83397-5-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MPHOSPH9 Recombinant antibody (83397-5-RR, Clone:240182D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83397-5-RR

    Affinity and Kinetic Characterization of 83397-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83397-5-RR against Human MPHOSPH9 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, rat brain tissue, NIH/3T3 cells, PC-12 cells, SH-SY5Y cells, mouse brain tissue
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83397-5-RR is MPHOSPH9 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MPHOSPH9 fusion protein Ag22228
Full Name M-phase phosphoprotein 9
Calculated Molecular Weight 1031 aa, 116 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC130440
Gene Symbol MPHOSPH9
Gene ID (NCBI) 10198
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MPHOSPH9, also known as MPP9, is a protein phosphorylated during mitosis. MPHOSPH9 negatively regulates cilia formation by recruiting the CP110-CEP97 complex at the distal end of the mother centriole in ciliary cells. MPHOSPH9 is a centrosome component that localizes to the distal and proximal ends of two centrioles(PMID: 30375385).

Protocols

Product Specific Protocols
WB protocol for MPHOSPH9 antibody 83397-5-RRDownload protocol
FC protocol for MPHOSPH9 antibody 83397-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols