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CD69 Monoclonal antibody

CD69 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D10A2

Cat no : 68827-1-Ig

Synonyms

Activation inducer molecule, AIM, BL AC/P26, CD69, CD69 molecule, CLEC2C, EA1, Early activation antigen CD69, GP32/28, MLR 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD69 Monoclonal antibody (68827-1-Ig)

    WB analysis using 68827-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68827-1-Ig (CD69 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inU-937 cells, THP-1 cells, HL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68827-1-Ig targets CD69 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen CD69 fusion protein Ag30963
Full Name CD69 molecule
Calculated Molecular Weight 23 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC007037
Gene Symbol CD69
Gene ID (NCBI) 969
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, and several subsets of tissue-resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in regulating immune responses (PMID: 15745855).

Protocols

Product Specific Protocols
WB protocol for CD69 antibody 68827-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols