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HIBCH Monoclonal antibody

HIBCH Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B7B8

Cat no : 68157-1-Ig

Synonyms

HIB CoA hydrolase, HIBCH, HIBYL COA H

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HIBCH Monoclonal antibody (68157-1-Ig)

    WB analysis using 68157-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68157-1-Ig (HIBCH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HIBCH Monoclonal antibody (68157-1-Ig)

    IF Staining of HepG2 using 68157-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (68157-1-Ig, Clone: 1B7B8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, K-562 cells, pig heart tissue, rabbit heart tissue, rat heart tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68157-1-Ig targets HIBCH in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HIBCH fusion protein Ag6473
Full Name 3-hydroxyisobutyryl-Coenzyme A hydrolase
Calculated Molecular Weight 43 kDa
Observed Molecular Weight38 kDa
GenBank Accession NumberBC067822
Gene Symbol HIBCH
Gene ID (NCBI) 26275
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).

Protocols

Product Specific Protocols
WB protocol for HIBCH antibody 68157-1-IgDownload protocol
IF protocol for HIBCH antibody 68157-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols