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GMEB2 Monoclonal antibody

GMEB2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2H10H8

Cat no : 68010-1-Ig

Synonyms

DNA binding protein p79PIF, GMEB 2, GMEB2, KIAA1269, P79PIF, PIF p79, PIF79

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GMEB2 Monoclonal antibody (68010-1-Ig)

    WB analysis using 68010-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of various lysates using GMEB2 Monoclonal antibody (68010-1-Ig)

    WB analysis using 68010-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using GMEB2 Monoclonal antibody (68010-1-Ig)

    WB analysis of MCF-7 using 68010-1-Ig

    WB result of GMEB2 antibody (68010-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GMEB2 transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using GMEB2 Monoclonal antibody (68010-1-Ig)

    WB analysis using 68010-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of MCF-7 cells using GMEB2 Monoclonal antibody (68010-1-Ig)

    WB analysis of MCF-7 using 68010-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using GMEB2 Monoclonal antibody (68010-1-Ig)

    WB analysis of RAW 264.7 using 68010-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, Jurkat cells, RAW 264.7 cells, HEK-293 cells, K-562 cells, PC-12 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68010-1-Ig targets GMEB2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GMEB2 fusion protein Ag31393
Full Name glucocorticoid modulatory element binding protein 2
Calculated Molecular Weight 530 aa, 56 kDa
Observed Molecular Weight68 kDa
GenBank Accession NumberBC036305
Gene Symbol GMEB2
Gene ID (NCBI) 26205
RRIDAB_2918757
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GMEB-2 (glucocorticoid modulatory element-binding protein 2), also known as PIF79 (Parvovirus initiation factor p79) or P79PIF, is a DNA-binding protein that plays a crucial role in modulating transcription upon activation by steroid hormones. GMEB-2 is ubiquitously expressed with preferential expression in developmentally important tissues.

Protocols

Product Specific Protocols
WB protocol for GMEB2 antibody 68010-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols