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  • Featured Product
  • KD/KO Validated

ASS1 Monoclonal antibody

ASS1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D1A11

Cat no : 66036-1-Ig

Synonyms

Argininosuccinate synthase, argininosuccinate synthetase 1, ASS, ASS1, Citrulline aspartate ligase, CTLN1

Validation Data Gallery

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  • Western Blot (WB) analysis of pig liver tissue using ASS1 Monoclonal antibody (66036-1-Ig)

    WB analysis of pig liver using 66036-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using ASS1 Monoclonal antibody (66036-1-Ig)

    WB analysis of L02 using 66036-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ASS1 Monoclonal antibody (66036-1-Ig)

    WB analysis of HeLa using 66036-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using ASS1 Monoclonal antibody (66036-1-Ig)

    WB analysis of human liver using 66036-1-Ig

    human liver tissue were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using ASS1 Monoclonal antibody (66036-1-Ig)

    IHC staining of human liver using 66036-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ASS1 Monoclonal antibody (66036-1-Ig)

    IHC staining of human liver using 66036-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ASS1 Monoclonal antibody (66036-1-Ig)

    IHC staining of human liver cancer using 66036-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ASS1 Monoclonal antibody (66036-1-Ig)

    IHC staining of human liver cancer using 66036-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using ASS1 Monoclonal antibody (66036-1-Ig)

    IHC staining of human testis using 66036-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ASS1 Monoclonal antibody (66036-1-Ig)

    IHC staining of human testis using 66036-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ASS1 Monoclonal antibody (66036-1-Ig)

    IHC staining of human liver using 66036-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ASS1 Monoclonal antibody (66036-1-Ig)

    IHC staining of human liver using 66036-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ASS1 Monoclonal antibody (66036-1-Ig)

    IF Staining of MCF-7 using 66036-1-Ig

    Immunofluorescent analysis of MCF-7 cells, using ASS1 antibody 66036-1-Ig at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

Tested Applications

Positive WB detected inpig liver tissue, HeLa cells, human liver tissue, L02 cells
Positive IHC detected inhuman liver tissue, human liver cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66036-1-Ig is ASS1 Fusion Protein expressed in E. coli.

Tested Reactivity human, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASS1 fusion protein Ag9314
Full Name argininosuccinate synthetase 1
Calculated Molecular Weight 412 aa, 47 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC009243
Gene Symbol ASS1
Gene ID (NCBI) 445
RRIDAB_11042883
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).

Protocols

Product Specific Protocols
WB protocol for ASS1 antibody 66036-1-IgDownload protocol
IHC protocol for ASS1 antibody 66036-1-IgDownload protocol
IF protocol for ASS1 antibody 66036-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FASEB J

Argininosuccinate synthetase 1 contributes to gastric cancer invasion and progression by modulating autophagy.

Authors - Chung-Ying Tsai
  • KD Validated
View Article
humanWB

Mol Pharmacol

MicroRNA-1291-5p Sensitizes Pancreatic Carcinoma Cells to Arginine Deprivation and Chemotherapy through the Regulation of Arginolysis and Glycolysis.

Authors - Mei-Juan Tu
View Article
humanWB

BMC Cancer

PMID: 25240403

Authors - Jiangbo Liu
View Article
humanWB

BMC Cancer

Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway by inhibiting cytosolic ferritin and inducing chromatin autophagy.

Authors - Qingyuan Feng
View Article