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THSD7A Polyclonal antibody

THSD7A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28524-1-AP

Synonyms

KIAA0960, THSD7A

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using THSD7A Polyclonal antibody (28524-1-AP)

    WB analysis using 28524-1-AP

    mouse kidney tissue was subjected to SDS PAGE followed by western blot with 28524-1-AP (THSD7A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:600-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28524-1-AP targets THSD7A in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THSD7A fusion protein Ag29107
Full Name thrombospondin, type I, domain containing 7A
Calculated Molecular Weight 185 kDa
Observed Molecular Weight240-250 kDa
GenBank Accession NumberNM_015204
Gene Symbol THSD7A
Gene ID (NCBI) 221981
RRIDAB_2918172
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thrombospondin type-1 domain-containing protein 7A (THSD7A) is a membrane-associated N-glycoprotein with a molecular weight of 250 kDa, which can be cleaved to release a 210-kDa soluble form (PMID: 22194972; 27214550). THSD7A has a role in angiogenesis. It mediates endothelial cell migration and tube formation (PMID: 2002048). THSD7A has been identified as a second autoantigen involved in adult idiopathic membranous nephropathy in addition to PLA2R1 (PMID: 25394321).

Protocols

Product Specific Protocols
WB protocol for THSD7A antibody 28524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Genomics Proteomics Bioinformatics

The Proteome Landscape of Human Placentas for Monochorionic Twins with Selective Intrauterine Growth Restriction

Authors - Xin-Lu Meng
View Article