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DBNDD2 Polyclonal antibody

DBNDD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27623-1-AP

Synonyms

C20orf35, CK1BP, DBNDD2, HSMNP1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using DBNDD2 Polyclonal antibody (27623-1-AP)

    WB analysis of mouse brain using 27623-1-AP

    mouse and rat brain tissues were subjected to SDS PAGE followed by western blot with 27623-1-AP (DBNDD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using DBNDD2 Polyclonal antibody (27623-1-AP)

    WB analysis of SH-SY5Y using 27623-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 27623-1-AP (DBNDD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using DBNDD2 Polyclonal antibody (27623-1-AP)

    IHC staining of rat brain using 27623-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27623-1-AP (DBNDD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using DBNDD2 Polyclonal antibody (27623-1-AP)

    IHC staining of rat brain using 27623-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27623-1-AP (DBNDD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inSH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27623-1-AP targets DBNDD2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DBNDD2 fusion protein Ag26356
Full Name dysbindin (dystrobrevin binding protein 1) domain containing 2
Calculated Molecular Weight 261 aa, 28 kDa
Observed Molecular Weight 28-29 kDa
GenBank Accession NumberBC001105
Gene Symbol DBNDD2
Gene ID (NCBI) 55861
RRIDAB_2880927
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DBNDD2 antibody 27623-1-APDownload protocol
IHC protocol for DBNDD2 antibody 27623-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cells

CK1BP Reduces α-Synuclein Oligomerization and Aggregation Independent of Serine 129 Phosphorylation.

Authors - Lea Elsholz
View Article