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  • Featured Product
  • KD/KO Validated

TMEM179 Polyclonal antibody

TMEM179 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24799-1-AP

Synonyms

C14orf90, TMEM179, TMEM179A, transmembrane protein 179, Transmembrane protein 179A

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using TMEM179 Polyclonal antibody (24799-1-AP)

    WB analysis of mouse liver using 24799-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24799-1-AP (TMEM179 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TMEM179 Polyclonal antibody (24799-1-AP)

    IHC staining of human breast cancer using 24799-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24799-1-AP (TMEM179 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TMEM179 Polyclonal antibody (24799-1-AP)

    IHC staining of human breast cancer using 24799-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24799-1-AP (TMEM179 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TMEM179 Polyclonal antibody (24799-1-AP)

    IHC staining of human breast cancer using 24799-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24799-1-AP (TMEM179 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TMEM179 Polyclonal antibody (24799-1-AP)

    IHC staining of human breast cancer using 24799-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24799-1-AP (TMEM179 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24799-1-AP is TMEM179 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM179 fusion protein Ag20502
Full Name transmembrane protein 179
Calculated Molecular Weight 233 aa, 26 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC148844
Gene Symbol TMEM179
Gene ID (NCBI) 388021
RRIDAB_2879732
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TMEM179 antibody 24799-1-APDownload protocol
IHC protocol for TMEM179 antibody 24799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Ecotoxicol Environ Saf

NAC antagonizes arsenic-induced neurotoxicity through TMEM179 by inhibiting oxidative stress in Oli-neu cells.

Authors - Zhixin He
  • KD Validated
View Article
humanWB

PLoS One

Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.

Authors - Kui Xiao
View Article