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C20orf160 Polyclonal antibody

C20orf160 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21493-1-AP

Synonyms

C20orf160, CCM2L

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using C20orf160 Polyclonal antibody (21493-1-AP)

    WB analysis of A431 using 21493-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 21493-1-AP (C20orf160 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using C20orf160 Polyclonal antibody (21493-1-AP)

    WB analysis of human placenta using 21493-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 21493-1-AP (C20orf160 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using C20orf160 Polyclonal antibody (21493-1-AP)

    IHC staining of human heart using 21493-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21493-1-AP (C20orf160 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using C20orf160 Polyclonal antibody (21493-1-AP)

    IHC staining of human heart using 21493-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21493-1-AP (C20orf160 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inA431 cells, human placenta tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21493-1-AP is C20orf160 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C20orf160 fusion protein Ag15841
Full Name chromosome 20 open reading frame 160
Calculated Molecular Weight 571 aa, 62 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC030254
Gene Symbol C20orf160
Gene ID (NCBI) 140706
RRIDAB_2878869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C20orf160 antibody 21493-1-APDownload protocol
IHC protocol for C20orf160 antibody 21493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols