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MRTO4 Polyclonal antibody

MRTO4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20194-1-AP

Synonyms

C1orf33, MRT4, MRTO4

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MRTO4 Polyclonal antibody (20194-1-AP)

    WB analysis of HEK-293 using 20194-1-AP

    WB result of MRTO4 antibody (20194-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRTO4 transfected HEK-293 cells.

  • Western Blot (WB) analysis of human skeletal muscle tissue using MRTO4 Polyclonal antibody (20194-1-AP)

    WB analysis of human skeletal muscle using 20194-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20194-1-AP (MRTO4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using MRTO4 Polyclonal antibody (20194-1-AP)

    WB analysis of mouse heart using 20194-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 20194-1-AP (MRTO4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using MRTO4 Polyclonal antibody (20194-1-AP)

    WB analysis of mouse heart using 20194-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 20194-1-AP (MRTO4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using MRTO4 Polyclonal antibody (20194-1-AP)

    WB analysis of mouse brain using 20194-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20194-1-AP (MRTO4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman skeletal muscle tissue, mouse heart tissue, mouse brain tissue, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20194-1-AP targets MRTO4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRTO4 fusion protein Ag14122
Full Name mRNA turnover 4 homolog (S. cerevisiae)
Calculated Molecular Weight 239 aa, 28 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC003013
Gene Symbol MRTO4
Gene ID (NCBI) 51154
RRIDAB_10697684
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MRTO4 antibody 20194-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols