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- KD/KO Validated
BMF Polyclonal antibody
BMF Polyclonal Antibody for IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, ELISA
Conjugate
Unconjugated
Cat no : 18298-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IP detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
Product Information
18298-1-AP targets BMF in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | Bcl2 modifying factor |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC069505 |
| Gene Symbol | BMF |
| Gene ID (NCBI) | 90427 |
| RRID | AB_2259162 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BMF belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities such as central regulators of apoptotic cell death. BMF contains a single BCL2 homology domain 3 (BH3), and has been shown to bind BCL2 proteins and function as an apoptotic activator. This protein is found to be sequestered to myosin V motors by its association with dynein light chain 2, which may be important for sensing intracellular damage and triggering apoptosis. Alternatively spliced transcript variants encoding different isoforms have been identified. BMF is widely expressed in tissues such as pancreas, liver and kidney, and in hematopoietic tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for BMF antibody 18298-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Sci RBMS2 Chemosensitizes Breast Cancer Cells to Doxorubicin by Regulating BMF Expression.
| ||
PLoS One Silencing prion protein in MDA-MB-435 breast cancer cells leads to pleiotropic cellular responses to cytotoxic stimuli. | ||
Aging Dis BMF-AS1/BMF Promotes Diabetic Vascular Calcification and Aging both In Vitro and In Vivo |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Chi (Verified Customer) (09-28-2019) | This antibody generally works well to detect BMF in western blot at a relatively high concentration.
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