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ICOS Polyclonal antibody

ICOS Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 13338-1-AP

Synonyms

Inducible T cell costimulator, inducible T cell co stimulator, CVID1, CD278, AILIM

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ICOS Polyclonal antibody (13338-1-AP)

    WB analysis of Jurkat using 13338-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13338-1-AP (ICOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using ICOS Polyclonal antibody (13338-1-AP)

    WB analysis of HL-60 using 13338-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13338-1-AP (ICOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ICOS Polyclonal antibody (13338-1-AP)

    IHC staining of human tonsillitis using 13338-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13338-1-AP (ICOS antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using ICOS Polyclonal antibody (13338-1-AP)

    IF Staining of human tonsillitis using 13338-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using ICOS antibody (13338-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, HL-60 cells
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13338-1-AP targets ICOS in WB, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ICOS fusion protein Ag4155
Full Name inducible T-cell co-stimulator
Calculated Molecular Weight 199 aa, 23 kDa
Observed Molecular Weight 28 kDa, 45-50 kDa
GenBank Accession NumberBC028210
Gene Symbol ICOS
Gene ID (NCBI) 29851
RRIDAB_2122590
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Inducible T cell co-stimulator (ICOS) is related to the CD28 superfamily and is highly expressed on activated T cells as well as regulatory T cells and is crucial for the survival and function of T cells, Th2 cell differentiation, and lung inflammatory responses. Binding ICOS to ICOS-ligand (ICOS-L) activates a cascade of intracellular signaling molecules that prevent apoptosis and lead to the production of cytokines such as IL-4 and IL-13. Catalog#13338-1-AP recognizes two bands of 25-30 kDa and 45-50 kDa, and the additional 45-50 kDa band may be due to  surface disulfide-linked homodimeric glycoprotein.

Protocols

Product Specific Protocols
WB protocol for ICOS antibody 13338-1-APDownload protocol
IF protocol for ICOS antibody 13338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols