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DYRK4 Polyclonal antibody

DYRK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12890-2-AP

Synonyms

DYRK4

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using DYRK4 Polyclonal antibody (12890-2-AP)

    WB analysis of mouse lung using 12890-2-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using DYRK4 Polyclonal antibody (12890-2-AP)

    WB analysis of Jurkat using 12890-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using DYRK4 Polyclonal antibody (12890-2-AP)

    WB analysis of mouse testis using 12890-2-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DYRK4 Polyclonal antibody (12890-2-AP)

    WB analysis of human brain using 12890-2-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using DYRK4 Polyclonal antibody (12890-2-AP)

    WB analysis of U-937 using 12890-2-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using DYRK4 Polyclonal antibody (12890-2-AP)

    IHC staining of human lymphoma using 12890-2-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 12890-2-AP (DYRK4 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inmouse lung tissue, human brain tissue, Jurkat cells, mouse testis tissue, U-937 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12890-2-AP targets DYRK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DYRK4 fusion protein Ag3515
Full Name dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4
Calculated Molecular Weight 520 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC031244
Gene Symbol DYRK4
Gene ID (NCBI) 8798
RRIDAB_2094086
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dual-specificity tyrosine phosphorylation regulated kinase 4(DYRK4), belongs to a conserved family of serine/threonine protein kinases. DYRK4 is thought to function in the regulation of cell differentiation and proliferation, survival, and in development. DYRK4 has 5 isoforms with molecular weight of 59, 72 and 73 kDa.

Protocols

Product Specific Protocols
WB protocol for DYRK4 antibody 12890-2-APDownload protocol
IHC protocol for DYRK4 antibody 12890-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols