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  • Featured Product
  • KD/KO Validated

KRAS Polyclonal antibody

KRAS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 12063-1-AP

Synonyms

KRAS4A, KRAS4B, pan RAS, GTPase KRas, EC:3.6.5.2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis using 12063-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of HEK-293 using 12063-1-AP

    WB result of KRAS antibody (12063-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KRAS transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis using 12063-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of mouse kidney using 12063-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of human brain using 12063-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of human kidney using 12063-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of rat kidney using 12063-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of rat liver using 12063-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using KRAS Polyclonal antibody (12063-1-AP)

    WB analysis of A549 using 12063-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using KRAS Polyclonal antibody (12063-1-AP)

    IP experiment of HeLa using 12063-1-AP

    IP result of anti-KRAS (IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:10000) with HeLa cells lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KRAS Polyclonal antibody (12063-1-AP)

    IHC staining of human lung cancer using 12063-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KRAS Polyclonal antibody (12063-1-AP)

    IHC staining of human lung cancer using 12063-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using KRAS Polyclonal antibody (12063-1-AP)

    IHC staining of human kidney using 12063-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using KRAS Polyclonal antibody (12063-1-AP)

    IHC staining of human kidney using 12063-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using KRAS Polyclonal antibody (12063-1-AP)

    IHC staining of human pancreas cancer using 12063-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using KRAS Polyclonal antibody (12063-1-AP)

    IHC staining of human pancreas cancer using 12063-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using KRAS Polyclonal antibody (12063-1-AP)

    IF Staining of mouse brain using 12063-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KRAS antibody (12063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using KRAS Polyclonal antibody (12063-1-AP)

    IF Staining of mouse brain using 12063-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KRAS antibody (12063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KRAS Polyclonal antibody (12063-1-AP)

    IF Staining of HeLa using 12063-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KRAS antibody (12063-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using KRAS Polyclonal antibody (12063-1-AP)

    FC experiment of HeLa using 12063-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KRAS (12063-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, A549 cells, rat liver tissue, HeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human kidney tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12063-1-AP targets KRAS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KRAS fusion protein Ag2700
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated Molecular Weight 188 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC013572
Gene Symbol KRAS
Gene ID (NCBI) 3845
RRIDAB_878040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.

Protocols

Product Specific Protocols
WB protocol for KRAS antibody 12063-1-APDownload protocol
IHC protocol for KRAS antibody 12063-1-APDownload protocol
IF protocol for KRAS antibody 12063-1-APDownload protocol
IP protocol for KRAS antibody 12063-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Biotechnol

Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors.

Authors - Christopher W Davies
View Article
humanWB

Cancer Discov

LZTR1 mutation mediates oncogenesis through stabilization of EGFR and AXL

Authors - Aram Ko
View Article
mouseWB,IHC

Cell Res

In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.

Authors - Zheng Fu
View Article
mouseWB

Cancer Discov

Systematic functional interrogation of rare cancer variants identifies oncogenic alleles.

Authors - Eejung Kim
View Article
humanWB

Mol Cell

The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.

Authors - Linyu Sun
View Article
humanWB

Mol Cell

Regulation of GTPase function by autophosphorylation.

Authors - Christian W Johnson
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Sophy (Verified Customer) (12-22-2022)

I have tried the KRas antibody from a lot of companies and this one from proteintech definitely has been working the best for me.

  • Applications: Western Blot
FH

Jonas (Verified Customer) (07-29-2022)

Antibody works as expected, dilution with 1:2000 was perfect and could even be increased to 1:5000; delivery was very fast, would recommend!!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
KRAS Antibody Western Blot validation (1:2000 dilution) in (Cat no:12063-1-AP)
FH

Usman Shah (Verified Customer) (11-29-2021)

Enables specific detection of KRAS in WBs.

  • Applications: Western Blot
FH

Kishor (Verified Customer) (12-13-2018)

I am very much satisfied with this antibody. I got good results for cells and rat liver tissue protein.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1, and Rat Liver tissues
FH

Christopher (Verified Customer) (12-11-2018)

Antibody is much better than comparable antibodies. Because of weak expression an very low differences between K-Ras/H-Ras/N-Ras it's quite difficult to detect only K-Ras in a specific way. That is also the problem of this antibody. Please look at my attached blot. 1. Fermentas prestained marker2. PANC-1 whole cell lysate3. PANC-1/EGFP-K-Ras4. HEK293/EGFP-K-Ras5. HEK293/EGFP-H-Ras6.HEK293/EGFP-N-Ras7. 20 ng recombinant K-Ras (His-tagged)Unfortunately, K-Ras (12063-1-AP) recognizes also H-Ras and in less amounts N-Ras. Looking at endogenous K-Ras detection, you have to keep in mind, that the upper band is K-Ras and the lower one H-Ras. Because of the fact, that N-Ras is only recognized in very low amounts (probably almost not on endogenous level) this antibody is great detecting endogenous K-Ras in whole cell lysates with some little problems

  • Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: pancreatic cancer cells (PANC-1) and HEK293 overexpressing EGFP-tagged K-Ras, H-Ras and N-Ras
KRAS Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:1000 dilution) in pancreatic cancer cells (PANC-1) and HEK293 overexpressing EGFP-tagged K-Ras, H-Ras and N-Ras (Cat no:12063-1-AP)