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  • Featured Product
  • KD/KO Validated

RASEF Polyclonal antibody

RASEF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 11569-1-AP

Synonyms

RAB45, Ras related protein Rab 45, RASEF

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using RASEF Polyclonal antibody (11569-1-AP)

    WB analysis of mouse testis using 11569-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11569-1-AP (RASEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using RASEF Polyclonal antibody (11569-1-AP)

    WB analysis of mouse heart using 11569-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11569-1-AP (RASEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RASEF Polyclonal antibody (11569-1-AP)

    IHC staining of human pancreas cancer using 11569-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11569-1-AP (RASEF antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inmouse testis tissue, mouse heart tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11569-1-AP targets RASEF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RASEF fusion protein Ag2149
Full Name RAS and EF-hand domain containing
Calculated Molecular Weight 740 aa, 83 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC023566
Gene Symbol RASEF
Gene ID (NCBI) 158158
RRIDAB_2238112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RASEF antibody 11569-1-APDownload protocol
IHC protocol for RASEF antibody 11569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF,IP

Mol Cancer Res

RASEF is a novel diagnostic biomarker and a therapeutic target for lung cancer.

Authors - Oshita Hideto H
  • KD Validated
View Article
humanIHC

Int J Clin Exp Pathol

High RASEF expression is associated with a significantly better prognosis in colorectal cancer

Authors - Xin Yu
View Article