Anticorps Monoclonal anti-SQLE

SQLE Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat

Applications

FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1C9A2

N° de cat : CL594-67206

Synonymes

ERG1, FLJ30795, SE, SQLE, squalene epoxidase, Squalene monooxygenase

---

Galerie de données de validation

• 

  FC experiment of HepG2 using CL594-67206

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SQLE (CL594-67206, Clone:1C9A2) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-67206 cible SQLE dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SQLE Protéine recombinante Ag3266
• Nom complet: squalene epoxidase
• Masse moléculaire calculée: 574 aa, 64 kDa
• Poids moléculaire observé: 50-64 kDa
• Numéro d’acquisition GenBank: BC017033
• Symbole du gène: SQLE
• Identification du gène (NCBI): 6713
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SQLE, also named as ERG1, SE and SM, belongs to the squalene monooxygenase family. It catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before cyclization into the basic steroid structure. SQLE may serve as a flux-controlling enzyme beyond 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, considered as rate limiting). It is also posttranslationally regulated by cholesterol-dependent proteasomal degradation. SQLE is subject to feedback regulation via cholesterol-induced degradation, which depends on its lipid-sensing N terminal regulatory domain. Truncation of SQLE occurs during its endoplasmic reticulum-associated degradation and requires the proteasome, which partially degrades the SQLE N-terminus and eliminates cholesterol-sensing elements within this region. The MW of SQLE is about 50-64 kDa. (PMID:21356516, PMID: 28972164)