DYRK4 Polyklonaler Antikörper

DYRK4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12890-2-AP

Synonyme

DYRK4

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 12890-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12890-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12890-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12890-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 12890-2-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 12890-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 12890-2-AP (DYRK4 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, humanes Hirngewebe, Jurkat-Zellen, Maushodengewebe, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12890-2-AP bindet in WB, IHC, ELISA DYRK4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DYRK4 fusion protein Ag3515
• Vollständiger Name: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4
• Berechnetes Molekulargewicht: 520 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC031244
• Gene symbol: DYRK4
• Gene ID (NCBI): 8798
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dual-specificity tyrosine phosphorylation regulated kinase 4(DYRK4), belongs to a conserved family of serine/threonine protein kinases. DYRK4 is thought to function in the regulation of cell differentiation and proliferation, survival, and in development. DYRK4 has 5 isoforms with molecular weight of 59, 72 and 73 kDa.