COQ9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

COQ9 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 14874-1-AP

Synonyme

C16orf49, COQ9, PSEC0129

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Galerie der Validierungsdaten

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  WB analysis using 14874-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14874-1-AP (COQ9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14874-1-AP

  WB result of COQ9 antibody (14874-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COQ9 transfected HEK-293 cells.

• 

  IP experiment of HeLa using 14874-1-AP

  IP Result of anti-COQ9 (IP:14874-1-AP, 3ug; Detection:14874-1-AP 1:500) with HeLa cells lysate 1840ug.

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  IHC staining of human liver cancer using 14874-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14874-1-AP (COQ9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14874-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14874-1-AP (COQ9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14874-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 14874-1-AP (COQ9 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Produktinformation

14874-1-AP bindet in WB, IP, IHC, IF, ELISA COQ9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COQ9 fusion protein Ag6645
• Vollständiger Name: coenzyme Q9 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC064946
• Gene symbol: COQ9
• Gene ID (NCBI): 57017
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COQ9, also named as C16orf49, HSPC326, and PSEC0129, is a 318 amino acid protein, which belongs to the COQ9 family. COQ9 localizes in the Mitochondrion and is involved in the biosynthesis of coenzyme Q COQ9 has abilities to improve left ventricular performance and to reduce myocardial infarct size and cardiomyocyte apoptosis.

Publikationen

Species	Application	Title
rat	IF	J Clin Invest; COQ6 mutations in human patients produce nephrotic syndrome with sensorineural deafness.; Authors - Heeringa Saskia F SF
human	WB	J Am Soc Nephrol; ADCK4 Deficiency Destabilizes the Coenzyme Q Complex, Which Is Rescued by 2,4-Dihydroxybenzoic Acid Treatment.; Authors - Eugen Widmeier
human	WB	J Med Genet; Human COQ4 deficiency: delineating the clinical, metabolic and neuroimaging phenotypes.; Authors - Lucia Laugwitz
human	WB	Front Physiol; Human COQ9 Rescues a coq9 Yeast Mutant by Enhancing Coenzyme Q Biosynthesis from 4-Hydroxybenzoic Acid and Stabilizing the CoQ-Synthome.; Authors - Cuiwen H He
human	WB	Mol Genet Metab; Detection of 6-demethoxyubiquinone in CoQ10 deficiency disorders: Insights into enzyme interactions and identification of potential therapeutics.; Authors - Diran Herebian ; KD Validated
human	IF	J Cell Biol; Coenzyme Q biosynthetic proteins assemble in a substrate-dependent manner into domains at ER-mitochondria contacts.; Authors - Kelly Subramanian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ying (Verified Customer) (03-31-2021)

This antibody works well for our westerns of cultured mouse cell extracts.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse macrophage