Alpha Tubulin Polyclonal antibody
Alpha Tubulin Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 14555-1-AP
Synonyms
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"Alpha Tubulin Antibodies" Comparison
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Tested Applications
| Positive WB detected in | mouse brain tissue, mouse cerebellum tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
14555-1-AP targets Alpha Tubulin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Alpha Tubulin fusion protein Ag6038 |
| Full Name | tubulin, alpha 1a |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC050637 |
| Gene Symbol | TUBA1A |
| Gene ID (NCBI) | 7846 |
| RRID | AB_2212258 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Alpha Tubulin antibody 14555-1-AP | Download protocol |
| IHC protocol for Alpha Tubulin antibody 14555-1-AP | Download protocol |
| IF protocol for Alpha Tubulin antibody 14555-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Neurosci Res Tau nuclear translocation is a leading step in tau pathology process through P53 stabilization and nucleolar dispersion. | ||
J Cell Sci ARF6 protects sister chromatid cohesion to ensure the formation of stable kinetochore-microtubule attachments. | ||
Biochim Biophys Acta Mol Basis Dis Dicer1 promotes Aβ clearance via blocking B2 RNA-mediated repression of apolipoprotein E. | ||
Exp Cell Res Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression. | ||
Cell Rep SFTSV Infection Induces BAK/BAX-Dependent Mitochondrial DNA Release to Trigger NLRP3 Inflammasome Activation. | ||
Nanomedicine Chemosensitization and mitigation of Adriamycin-induced cardiotoxicity using combinational polymeric micelles for co-delivery of quercetin/resveratrol and resveratrol/curcumin in ovarian cancer. |
