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SOAT1/ACAT1 Polyclonal antibody

SOAT1/ACAT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13202-1-AP

Synonyms

ACACT, ACACT1, ACAT, ACAT 1, ACAT1, Cholesterol acyltransferase 1, RP11 215I23.2, SOAT, SOAT1, STAT, sterol O acyltransferase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SOAT1/ACAT1 Polyclonal antibody (13202-1-AP)

    WB analysis using 13202-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13202-1-AP (SOAT1/ACAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HuH-7 cells, SW480 cells, THP-1 cells, mouse liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13202-1-AP targets SOAT1/ACAT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SOAT1/ACAT1 fusion protein Ag3944
Full Name sterol O-acyltransferase 1
Calculated Molecular Weight 550 aa, 65 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC028940
Gene Symbol SOAT1/ACAT1
Gene ID (NCBI) 6646
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sterol O-acyltransferase 1 (SOAT1/ACAT1) catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol (PubMed:16154994,16647063, 32433614, 32944968). It plays a role in lipoprotein assembly and dietary cholesterol absorption (PubMed:16154994, 9020103). SOAT1 has three isoforms with molecular weights of 65, 58, and 57 kDa.

Protocols

Product Specific Protocols
WB protocol for SOAT1/ACAT1 antibody 13202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols