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  • KD/KO Validated

PSPH Polyclonal antibody

PSPH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14513-1-AP

Synonyms

PSPase, PSP, O-phosphoserine phosphohydrolase, L-3-phosphoserine phosphatase, EC:3.1.3.3



Tested Applications

Positive WB detected inA375 cells, rat liver tissue, U-87 MG cells, HL-60 cells, MCF-7 cells, SK-BR-3 cells
Positive IP detected inHL-60 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14513-1-AP targets PSPH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSPH fusion protein Ag5972
Full Name phosphoserine phosphatase
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC063614
Gene Symbol PSPH
Gene ID (NCBI) 5723
RRIDAB_2171464
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSPH (phosphoserine phosphatase) is an enzyme, which is involved in the process of L-serine biosynthesis. PSPH mainly plays role in multiple aspects of cell behaviours such as proliferation and differentiation by producing precursors for the biosynthesis of diverse compounds including neurotransmitters, glycolipids and thymidine (PMID: 11237721, PMID: 19963421). Additionally, augmented PSPH level is correlated with the prognosis in multiple cancers including cutaneous squamous cell carcinoma (PMID: 21726982), breast cancer (PMID: 28931725), non-small cell lung cancer (PMID: 30662358), colorectal cancer (PMID: 24146633) and hepatocellular carcinoma (PMID: 25793315).

Protocols

Product Specific Protocols
WB protocol for PSPH antibody 14513-1-APDownload protocol
IHC protocol for PSPH antibody 14513-1-APDownload protocol
IF protocol for PSPH antibody 14513-1-APDownload protocol
IP protocol for PSPH antibody 14513-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Science

mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.

Authors - Issam Ben-Sahra
humanWB

Cell Metab

Epstein-Barr-Virus-Induced One-Carbon Metabolism Drives B Cell Transformation.

Authors - Liang Wei Wang
humanWB

Cell Metab

Serine metabolism antagonizes antiviral innate immunity by preventing ATP6V0d2-mediated YAP lysosomal degradation.

Authors - Long Shen
humanWB,IP

Nat Commun

PHGDH arginine methylation by PRMT1 promotes serine synthesis and represents a therapeutic vulnerability in hepatocellular carcinoma

Authors - Kui Wang
humanWB

Nat Commun

Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence

Authors - Yinsheng Wu
humanWB

Mol Cell

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Authors - Youming Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Hua (Verified Customer) (11-30-2022)

Good antibody for WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HepG2 cells, mouse liver, and mouse brain
PSPH Antibody Western Blot validation (1:1000 dilution) in HepG2 cells, mouse liver, and mouse brain (Cat no:14513-1-AP)