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P27; KIP1 Polyclonal antibody

Name: P27; KIP1 Polyclonal antibody
Brand: Proteintech
SKU: 25614-1-AP
Rating: 4.33 (3 reviews)

P27; KIP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB,IP,IHC,IF,FC,ELISA

Conjugate

Unconjugated

Publications

183

Reviews (3)

Cat no : 25614-1-AP

Synonyms

CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27; KIP1, P27KIP1

Validation Data Gallery

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AllWBIPIHCIFFC

WB analysis using 25614-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25614-1-AP (P27; KIP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 25614-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25614-1-AP (P27; KIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 25614-1-AP
WB result of P27; KIP1 antibody (25614-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P27; KIP1 transfected HeLa cells.
WB analysis using 25614-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25614-1-AP (P27; KIP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 25614-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25614-1-AP (P27; KIP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 25614-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25614-1-AP (P27; KIP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 25614-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25614-1-AP (P27; KIP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of NIH/3T3 using 25614-1-AP
IP result of anti-P27; KIP1 (IP:25614-1-AP, 4ug; Detection:25614-1-AP 1:1000) with NIH/3T3 cells lysate 3440 ug.
IHC staining of human breast cancer using 25614-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25614-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 25614-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25614-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 25614-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25614-1-AP (P27; KIP1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 25614-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25614-1-AP (P27; KIP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 25614-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25614-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 25614-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25614-1-AP (P27; KIP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 25614-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25614-1-AP (P27; KIP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 25614-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25614-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 25614-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 25614-1-AP (P27; KIP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse testis using 25614-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using P27; KIP1 antibody (25614-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse testis using 25614-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using P27; KIP1 antibody (25614-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of MCF-7 using 25614-1-AP
1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human P27; KIP1 (25614-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 25614-1-AP

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Tested Applications

Positive WB detected in	HeLa cells, NIH/3T3 cells, HepG2 cells, MCF-7 cells, Jurkat cells
Positive IP detected in	NIH/3T3 cells
Positive IHC detected in	human breast cancer tissue, human lung cancer tissue, human colon cancer tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells, mouse testis tissue
Positive FC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
Flow Cytometry (FC)	FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 176 publications below
IHC	See 14 publications below
IF	See 4 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

25614-1-AP targets P27; KIP1 in WB,IP,IHC,IF,FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, sheep, canine, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	P27; KIP1 fusion protein Ag22582
Full Name	cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Calculated Molecular Weight	198 aa, 22 kDa
Observed Molecular Weight	27 kDa
GenBank Accession Number	BC001971
Gene Symbol	CDKN1B
Gene ID (NCBI)	1027
RRID	AB_2880161
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.

Protocols

Product Specific Protocols
WB protocol for P27; KIP1 antibody 25614-1-AP	Download protocol
IHC protocol for P27; KIP1 antibody 25614-1-AP	Download protocol
IF protocol for P27; KIP1 antibody 25614-1-AP	Download protocol
IP protocol for P27; KIP1 antibody 25614-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Gastroenterology Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6. Authors - Yuhong Luo View Article
human	WB	Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. Authors - Pengli Zheng View Article
human	WB	Mol Cancer LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer. Authors - Xi Jin View Article
human	WB	Mol Cancer CircGPR137B/miR-4739/FTO feedback loop suppresses tumorigenesis and metastasis of hepatocellular carcinoma. Authors - Lianyong Liu View Article
human	WB	Small Synergistic Silencing of Skp2 by siRNA Self-Assembled Nanoparticles as a Therapeutic Strategy for Advanced Prostate Cancer. Authors - Hong Liang View Article
human	WB	Cell Death Dis UBE2E2 enhances Snail-mediated epithelial-mesenchymal transition and Nrf2-mediated antioxidant activity in ovarian cancer Authors - Xiaoling Hong View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Malak (Verified Customer) (04-22-2021)	The optimal incubation period is 1.5 hr at room temperature. Avoid incubating the antibody overnight as it may result in aspecific signals. Applications: Western Blot Primary Antibody Dilution: 1/2000 Cell Tissue Type: Hepatic cancer cell extract
FH Susmita (Verified Customer) (09-17-2020)	Antibody is working very good for WB Applications: Western Blot Primary Antibody Dilution: 1:1000 WB;
FH Joshua (Verified Customer) (07-27-2019)	Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Decent staining with some background in rabbit cells. Nuclear localization as expected. Applications: Immunofluorescence, Primary Antibody Dilution: 1:500 Cell Tissue Type: Rabbit Corneal Epithelial Cells P27; KIP1 Antibody Immunofluorescence, validation (1:500 dilution) in Rabbit Corneal Epithelial Cells (Cat no:25614-1-AP)

P27; KIP1 Polyclonal antibody

P27; KIP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis using 25614-1-AP

WB analysis using 25614-1-AP

WB analysis of HeLa using 25614-1-AP

WB analysis using 25614-1-AP

WB analysis using 25614-1-AP

WB analysis of HeLa using 25614-1-AP

WB analysis of NIH/3T3 using 25614-1-AP

IP experiment of NIH/3T3 using 25614-1-AP

IHC staining of human breast cancer using 25614-1-AP

IHC staining of human breast cancer using 25614-1-AP

IHC staining of human ovary tumor using 25614-1-AP

IHC staining of human ovary tumor using 25614-1-AP

IHC staining of human lung cancer using 25614-1-AP

IHC staining of human colon cancer using 25614-1-AP

IHC staining of human colon cancer using 25614-1-AP

IHC staining of human lung cancer using 25614-1-AP

IF Staining of HepG2 using 25614-1-AP

IF Staining of mouse testis using 25614-1-AP

IF Staining of mouse testis using 25614-1-AP

FC experiment of MCF-7 using 25614-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6.

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.

CircGPR137B/miR-4739/FTO feedback loop suppresses tumorigenesis and metastasis of hepatocellular carcinoma.

Synergistic Silencing of Skp2 by siRNA Self-Assembled Nanoparticles as a Therapeutic Strategy for Advanced Prostate Cancer.

UBE2E2 enhances Snail-mediated epithelial-mesenchymal transition and Nrf2-mediated antioxidant activity in ovarian cancer

Reviews

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