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  • Featured Product
  • KD/KO Validated

MAX Polyclonal antibody

MAX Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10426-1-AP

Synonyms

bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8, MAX, MYC associated factor X, orf1, Protein max

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MAX Polyclonal antibody (10426-1-AP)

    WB analysis of HEK-293 using 10426-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10426-1-AP (MAX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MAX Polyclonal antibody (10426-1-AP)

    WB analysis of HEK-293 using 10426-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10426-1-AP (MAX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using MAX Polyclonal antibody (10426-1-AP)

    WB analysis of PC-3 using 10426-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10426-1-AP (MAX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10426-1-AP targets MAX in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAX fusion protein Ag0680
Full Name MYC associated factor X
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC003525
Gene Symbol MAX
Gene ID (NCBI) 4149
RRIDAB_2141660
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.

Protocols

Product Specific Protocols
WB protocol for MAX antibody 10426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cancer Cell

MAX Functions as a Tumor Suppressor and Rewires Metabolism in Small Cell Lung Cancer.

Authors - Arnaud Augert
  • KO Validated
View Article
humanWB

Sci Adv

A MYC inhibitor selectively alters the MYC and MAX cistromes and modulates the epigenomic landscape to regulate target gene expression.

Authors - Austin G Holmes
View Article
humanWB

Nat Commun

PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.

Authors - Xianchun Lan
View Article
humanWB,ChIP

Elife

Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness.

Authors - Haritha Mathsyaraja
View Article
humanWB

Cell Commun Signal

Novel dual-targeting c-Myc inhibitor D347-2761 represses myeloma growth via blocking c-Myc/Max heterodimerization and disturbing its stability.

Authors - Ruosi Yao
View Article
humanchIP

J Biol Chem

Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1.

Authors - Jianyin Long
View Article