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JTV1 Polyclonal antibody

JTV1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10424-1-AP

Synonyms

AIMP2, JTV1, JTV1 gene, P38, PRO0992, Protein JTV 1



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, NIH/3T3 cells, K-562 cells, mouse liver tissue, L02 cells, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human kidney tissue, mouse cerebellum tissue, mouse testis tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10424-1-AP targets JTV1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JTV1 fusion protein Ag0675
Full Name JTV1 gene
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC002853
Gene Symbol JTV1
Gene ID (NCBI) 7965
RRIDAB_513880
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JTV1, also named p38, AIMP2, was first knew as a factor associated with macromolecular protein complex, which consists several different aminoacyl-tRNA synthetases. JTV1 is a scaffold protein required for the assembly and stability of the multi-tRNA synthetase complex. JTV1 could act as a mediator of TGF-beta signaling and its functional importance in the control of MYC during lung differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor. This is a rabbit polyclonal antibody raised against full-length of JTV1. There're two isoforms of JTV1 with MW about 27 kDa and 35 kDa,

Protocols

Product Specific Protocols
WB protocol for JTV1 antibody 10424-1-APDownload protocol
IHC protocol for JTV1 antibody 10424-1-APDownload protocol
IF protocol for JTV1 antibody 10424-1-APDownload protocol
IP protocol for JTV1 antibody 10424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Global landscape of HIV-human protein complexes.

Authors - Stefanie Jäger
mouseWB,IF

Nat Neurosci

Parthanatos mediates AIMP2-activated age-dependent dopaminergic neuronal loss.

Authors - Lee Yunjong Y
humanWB

J Exp Clin Cancer Res

RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization

Authors - Shuang Tao
humanWB,IF

Nat Commun

Molecular determinants of selective clearance of protein inclusions by autophagy.

Authors - Wong Esther E
mouseWB

Proc Natl Acad Sci U S A

Role for the obesity-related FTO gene in the cellular sensing of amino acids.

Authors - Gulati Pawan P
ratWB

Proc Natl Acad Sci U S A

Phosphorylation by the c-Abl protein tyrosine kinase inhibits parkin's ubiquitination and protective function.

Authors - Ko Han Seok HS

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

KRishnendu (Verified Customer) (02-27-2020)

Very strong antibody with no contaminant bands.

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 1:2000 for WB; 1:100 for IP
  • Cell Tissue Type: HEK293T