Adiponectin Monoclonal antibody

Adiponectin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

5D8A7

Cat no : 66239-1-Ig

Synonyms

ACDC, ACRP30, adiponectin, ADIPOQ, ADIPQTL1, ADPN, APM 1, APM1, GBP28, Gelatin binding protein



Tested Applications

Positive WB detected inhuman adipose tissue
Positive IHC detected inmouse skeletal muscle tissue, mouse skin tissue, human placenta tissue, human prostate cancer tissue, mouse brown adipose tissue, rat brown adipose tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in3T3-L1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:16000-1:64000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

66239-1-Ig targets Adiponectin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Adiponectin fusion protein Ag17383
Full Name adiponectin, C1Q and collagen domain containing
Calculated Molecular Weight 244 aa, 26 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC096308
Gene Symbol ADIPOQ
Gene ID (NCBI) 9370
RRIDAB_2881628
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.

Protocols

Product Specific Protocols
WB protocol for Adiponectin antibody 66239-1-IgDownload protocol
IHC protocol for Adiponectin antibody 66239-1-IgDownload protocol
IF protocol for Adiponectin antibody 66239-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Physiol

Rapid responses of adipocytes to iron overload increase serum TG level by decreasing adiponectin.

Authors - Yuxiao Tang
mouseWB

Food Funct

Quercetin regulates skeletal muscle fiber type switching via adiponectin signaling.

Authors - Xiaoling Chen
pigWB

Anim Biotechnol

Effect of dietary leucine supplementation on skeletal muscle fiber type transformation in weaning piglets

Authors - Xiaoling Chen
pigWB

Anim Biotechnol

Leucine regulates porcine muscle fiber type transformation via adiponectin signaling pathway.

Authors - Lu Xiang