Various lysates were subjected to SDS PAGE followed by western blot with 60303-1-Ig (SIRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.
WB result of SIRT1antibody (60303-1-Ig, 1:500) with si-Control and si-SIRT1 transfected HEK-293 cells. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 60303-1-Ig (SIRT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.
IP result of anti-SIRT1 (IP:60303-1-Ig, 5ug; Detection:60303-1-Ig 1:1000) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60303-1-Ig (SIRT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60303-1-Ig(SIRT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.