Hela cells immunostained with Histone-Lable-ATTO488, 1:400. Scale bar, 10 um.

Hela cells immunostained with Histone-Lable-ATTO488, 1:400. Scale bar, 10 um.

Hela cells immunostained with Histone-Lable-ATTO488, 1:400. Scale bar, 10 um.

ChromoTek Histone-Label is a convenient, ready to use, and high-performing chromatin staining probe with low background levels and that differentiates between euchromatin and heterochromatin. HeLa cells stained in parallel with Histone-Label (1.25 mg/L) and a monoclonal rabbit anti-H2A IgG / anti-rabbit Alexa568 secondary antibody. Histone-Label co-localizes with conventional antibody staining, however Histone-Label better penetrates into the compactly packed nuclei than the anti-H2A IgG and secondary IgGs, which are one order of magnitude larger than the Histone-Label: see more green signal from Histone-Label at center of nuclei and more red signal from anti-H2A IgG on surface/edge of nuclei of merge image on very right.

MDCK cells were immunostained with Vimentin-Booster (in green), cell nuclei were counterstained with DAPI (in blue).

Immunofluorescence with anti-HA-Label, CoraLite ® Plus 488 (hbCL488, 1:500, green) visualizes HA-tagged Vimentin, transiently expressed in HeLa cells. Nuclei are stained with DAPI (blue). Confocal images were were acquired with a 63x oil objective and post-processed.

Multiplex Western Blot with HA-Label (hbCL488, 1:500) targeting HA-labeled TOM70 (blue) and CoraLite®647-conjugated GAPDH (Human Specific) Recombinant antibody (red) 1:500; PTG CL647-80570. Tested on HEK293T lysate and HEK293T transfected with TOM70-HA (Protein ladder: PL00002)

Immunofluorescence with anti-HA-Label, CoraLite ® Plus 555 (hbCL555, 1:500, orange) visualizes HA-tagged Vimentin, transiently expressed in HeLa cells. Nuclei are stained with DAPI (cyan). Confocal images were were acquired with a 63x oil objective and post-processed.

Multiplex Western Blot with HA-Label (hbCL555, 1:500) targeting HA-labeled TOM70 (blue) and CoraLite®647-conjugated GAPDH (Human Specific) Recombinant antibody (red) 1:500; PTG CL647-80570. Tested on HEK293T lysate and HEK293T transfected with TOM70-HA (Protein ladder: PL00002)

Immunofluorescence with anti-HA-Label, CoraLite ® Plus 647 (hbCL647, 1:500, magenta) visualizes HA-tagged Vimentin, transiently expressed in HeLa cells. Nuclei are stained with DAPI (cyan). Confocal images were were acquired with a 63x oil objective and post-processed.

Western Blot with HA-Label (hbCL488, 1:500) targeting HA-labeled TOM70 (black). Tested on HEK293T lysate and HEK293T transfected with TOM70-HA (Protein ladder: PL00002)