HCT 116 (WT and SYT1 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 14511-1-AP incubated at 1:500 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

mouse cerebellum tissue was subjected to SDS PAGE followed by western blot with 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human liver cancer using 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:100 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human liver cancer using 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:100 (under 40x lens).

HCT 116 lysates prepared and IP of SYT1 performed using 1.0 μg of 14511-1-AP coupled to protein A- Sepharose beads. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

IP Result of anti-Synaptotagmin-1 (IP:14511-1-AP, 4ug; Detection:14511-1-AP 1:500) with mouse testis tissue lysate 4000ug.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

HCT 116 WT cells (yellow outline) and SYT1 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 14511-1-AP at 1:500 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human Synaptotagmin-1 (CL488-68043, Clone:1H1H2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

SYT1 fusion protein Ag5970 SDS-PAGE

SYT1 fusion protein Ag6071 SDS-PAGE

Various lysates were subjected to SDS PAGE followed by western blot with 67688-1-Ig (ESYT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 67688-1-Ig (FAM62A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ESYT1 antibody (67688-1-Ig, Clone: 2C12E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 67688-1-Ig (ESYT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67688-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 67688-1-Ig (FAM62A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67688-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ESYT1 antibody (67688-1-Ig, Clone: 2C12E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67688-1-PBS in a different storage buffer formulation.