1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse CD117 (65054-1-Ig, Clone: 2B8) or 0.5 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2), FITC anti-rat IgG2b Antibody at dilution 1:100, and 0.5 ug APC Anti-Mouse CD45R (B220) (RA3-6B2) (APC-65139, Clone: RA3-6B2). Cells were not fixed.