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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-19 protein

Activity(Primary)

200-1000 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1332

Synonyms

NG.1, Melanoma differentiation-associated protein-like protein, Interleukin-19, MDA1, ZMDA1

Validation Data Gallery

  • Purity of recombinant human IL-19 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-19 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-19 (HZ-1332) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were treated with increasing concentrations of recombinant human PDGFbb under low serum conditions for 72hrs. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 200-1000 ng/mL.

    Recombinant human IL-19 (HZ-1332) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were treated with increasing concentrations of recombinant human PDGFbb under low serum conditions for 72hrs. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 200-1000 ng/mL.

Technical Specifications

GeneID 29949
Species Human
Expression HEK293
Activity(Primary) 200-1000 ng/mL EC50
Purity >95%
Endotoxin<1 EU/μg
Accession NumberQ9UHD0
Molecular Mass 25-32 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Interleukin-19 (IL-19) is part of a smaller subfamily of interleukins that also includes IL-20, IL-22, and IL-24. These cytokines are all classified by their shared ability to bind and signal through the IL-20 receptor. IL-19 primarily exerts anti-inflammatory effects. It upregulates the production of IL-10 in PBMCs, particularly monocytes and dendritic cells and promotes the secretion of Th2-associated cytokine secretion from CD4+ T cells. It is thought to favor the polarization of naïve T cells into Th2s over Th1s, yielding a more anti-inflammatory phenotype. IL-19 also has several anti-inflammatory roles in vascular diseases, with its levels often increased in these instances to protect arteries from excessive damage (PMID: 29681905, 15827959).

Synonyms

NG.1, Melanoma differentiation-associated protein-like protein, Interleukin-19, MDA1, ZMDA1