ChromoTek pan-RFP Polyclonal antibody
Rabbit polyclonal antibody to pan-Red Fluorescent Protein
Host/IsoType
Rabbit / IgG
Specificity
RFP and RFP derivates
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : pabr1
Synonyms
Validation Data Gallery
Product Information
Rabbit polyclonal antibody to pan-Red Fluorescent Protein
Description | Rabbit polyclonal antibody to Red Fluorescent Protein |
Tested Applications | WB, IF, ELISA |
Tested Reactivity | Recombinant proteins, stably and transiently transfected cell lines; red fluorescent proteins from Discosoma (dsRed, mCherry, tdTomato, mPlum); fluorescent proteins from Entacmaea lineages (TagRFP, TagBFP, mKate2, mRuby2), synthetic red fluorescent protein mScarlet. |
Recommended Dilution | Western blot: 1:1,000 IF: 1:400-1:800 ELISA: N/A |
Host/IsoType | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Red Fluorescent Proteins (RFPs), a mixture of several representatives from different lineages |
Calculated molecular weight | 26kDa |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
RRID | N/A |
Storage Buffer | PBS with 50% glycerol and 0.05% NaN3 |
Storage Condition | Shipped on ice. Store at -20°C. Stable for 1 year after shipment. |
Background | Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, belonging to distinct protein lineages originating from different species. RFPs are widely used in biomedical research to tag proteins of interest in order to, for example, monitor their subcellular localization. PABR1 antibody can detect a broad range of red fluorescent proteins from different species. This antibody detects dsRed-derivatives from Discosoma sp., including mCherry, mRFP, tdTomato, mPlum. PABR1 antibody also detects representatives of different FP lineages from Entacmaea sp., such as eqFP578-derivatives including TagRFP, TagBFP, mKate2, as well as eqFP611-derivatives including mRuby2. PABR1 also detects the red fluorescent protein mScarlet, engineered from a synthetic template. For more information on different families of fluorescent proteins, please refer to a free resource www.fpbase.org/lineage/. |
Documentation
SDS |
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PABR1_SDS_pan-RFP antibody rabbit polyclonal [PABR1] (EN) |
Protocol |
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pabr1-WB Protocol |
Datasheet |
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pabr1_Datasheet_RFP antibody [pabr1] |