Nectin-4/PVRL4 Polyclonal antibody
Nectin-4/PVRL4 Polyclonal Antibody for WB, IF, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF, FC, IHC, ELISA
Conjugate
Unconjugated
Cat no : 21903-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, HeLa cells, NIH/3T3 cells, A2780 cells |
Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | NIH/3T3 cells |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 3 publications below |
IHC | See 2 publications below |
Product Information
21903-1-AP targets Nectin-4/PVRL4 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Nectin-4/PVRL4 fusion protein Ag14127 |
Full Name | poliovirus receptor-related 4 |
Calculated Molecular Weight | 510 aa, 55 kDa |
Observed Molecular Weight | 43 kDa, 66 kDa |
GenBank Accession Number | BC010423 |
Gene Symbol | PVRL4 |
Gene ID (NCBI) | 81607 |
RRID | AB_2878941 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nectin-4, or PVRL4, belongs to the family of cell adhesion molecules that regulate the formation of adherence functions in epithelial cells through the AF-6 afadin scaffold molecule. Nectin-4 is reported as a histological and serological marker for breast carcinoma. Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).
Protocols
Product Specific Protocols | |
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WB protocol for Nectin-4/PVRL4 antibody 21903-1-AP | Download protocol |
IHC protocol for Nectin-4/PVRL4 antibody 21903-1-AP | Download protocol |
IF protocol for Nectin-4/PVRL4 antibody 21903-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Oncol MiR-31 and miR-128 regulates poliovirus receptor-related 4 mediated measles virus infectivity in tumors. | ||
Int J Urol Chemotherapy with gemcitabine and cisplatin downregulates tumor expression level of nectin-4 in a syngeneic model of murine MBT2 urothelial cancer cell line and C3H mice | ||
Oncol Lett Upregulation of nectin-4 is associated with ITGB1 and vasculogenic mimicry and may serve as a predictor of poor prognosis in colorectal cancer. | ||
Cell Biosci Interferon-stimulated gene PVRL4 broadly suppresses viral entry by inhibiting viral-cellular membrane fusion
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Morgan (Verified Customer) (08-09-2021) | I had a lot of trouble using this PVRL4 antibody on western blots of 2 ovarian cancer cell lines; OV8 and OV90. I kept getting prominent bands at 250 kda. I tried cutting the 250 band to see if that would help, but it did not make the signal any better in the band(s) below. Any insight on this would be greatly appreciated. Thank you.
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