fetuin-B Polyclonal antibody

fetuin-B Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18052-1-AP

Synonyms

16G2, FETUB, fetuin B, Fetuin like protein IRL685, fetuin-B, Gugu, IRL685

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Validation Data Gallery

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  WB analysis of human plasma using 18052-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 18052-1-AP (fetuin-B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 18052-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 18052-1-AP (fetuin-B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human plasma using 18052-1-AP

  IP result of anti-fetuin-B (IP:18052-1-AP, 4ug; Detection:18052-1-AP 1:2000) with human plasma lysate 4000 ug.

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  IHC staining of human liver cancer using 18052-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18052-1-AP (fetuin-B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18052-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18052-1-AP (fetuin-B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 18052-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 18052-1-AP (fetuin-B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 18052-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 18052-1-AP (fetuin-B antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human blood tissue, human plasma
• Positive IP detected in: human plasma tissue
• Positive IHC detected in: human liver cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18052-1-AP targets fetuin-B in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: fetuin-B fusion protein Ag12562
• Full Name: fetuin B
• Calculated Molecular Weight: 382 aa, 42 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC074734
• Gene Symbol: FETUB
• Gene ID (NCBI): 26998
• RRID: AB_2103181
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fetuin-B is a protein that in humans is encoded by the FETUB gene. The protein encoded by this gene is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the ins and hepatocyte growth factor receptors, and response to systemic inflammation. It may be a protease inhibitor required for egg fertilization.