CoraLite® Plus 488-conjugated c-Met (Cytoplasmic) Polyclonal antibody

• Featured Product
• KD/KO Validated

c-Met (Cytoplasmic) Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, canine, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-25869

Synonyms

AUTS9, c Met, HGF receptor, HGF/SF receptor, HGFR, MET, Proto oncogene c Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine protein kinase Met

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Validation Data Gallery

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  FC experiment of HeLa using CL488-25869

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human c-Met (Cytoplasmic) (CL488-25869) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-25869 targets c-Met (Cytoplasmic) in FC (Intra) applications and shows reactivity with human, canine, mouse, rat samples.

• Tested Reactivity: human, canine, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: c-Met (Cytoplasmic) fusion protein Ag23140
• Full Name: met proto-oncogene (hepatocyte growth factor receptor)
• Calculated Molecular Weight: 1390 aa, 155 kDa
• Observed Molecular Weight: 145 kDa
• GenBank Accession Number: BC130420
• Gene Symbol: MET
• Gene ID (NCBI): 4233
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

c-Met (also named as MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to HGF ligand. c-Met regulates many physiological processes including proliferation, scattering, morphogenesis and survival. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Overexpression and/or mutation of c-Met has been reported in various human malignancies, including lung cancer, breast cancer, head and neck cancer, gastric cancer, colorectal cancer, bladder cancer, uterine cervix carcinoma, and esophageal carcinoma, c-Met could serve as an important therapeutic target (PMID: 26036285). The c-met receptor is a 190-kD glycoprotein consisting of a 145-kD membrane-spanning beta chain and a 50-kD alpha chain (PMID: 7806559). In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.