ZBTB20 Polyclonal antibody

ZBTB20 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 23987-1-AP

Synonyms

DPZF, HOF, ODA 8S, ZBTB20, Zinc finger protein 288, ZNF288

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Validation Data Gallery

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  WB analysis using 23987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 23987-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 23987-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 23987-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23987-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23987-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23987-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 23987-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 23987-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23987-1-AP (ZBTB20 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 23987-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23987-1-AP (ZBTB20 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of mouse brain using 23987-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ZBTB20 antibody (23987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, Jurkat cells, L02 cells, mouse brain tissue, mouse liver tissue, RAW 264.7 cells, rat liver tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

23987-1-AP targets ZBTB20 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZBTB20 fusion protein Ag21140
• Full Name: zinc finger and BTB domain containing 20
• Calculated Molecular Weight: 668 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC029041
• Gene Symbol: ZBTB20
• Gene ID (NCBI): 26137
• RRID: AB_2879389
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZBTB20 (zinc finger and BTB domain containing 20), also known as HOF, DPZF, ODA-8S or ZNF288, is a 741 amino acid protein that localizes to the nucleus and contains one BTB (POZ) domain and five C2H2-type zinc fingers. Expressed in thymus, spleen, lymph node and fetal liver, ZBTB20 exists as either a monomer or a homodimer that is thought to function as a transcription factor, playing a role in hematopoiesis, oncogenesis and immune responses. Multiple isoforms of ZBTB20 exist due to alternative splicing events.

Publications

Species	Application	Title
	IF	Cell Prolif; In vivo development and single-cell transcriptome profiling of human brain organoids.; Authors - Shichao Huang
mouse	WB,IHC	J Inflamm Res; Aberrant Fluid Shear Stress Contributes to Articular Cartilage Pathogenesis via Epigenetic Regulation of ZBTB20 by H3K4me3.; Authors - Yu Jin
mouse	WB	Wellcome Open Res; The integration site of the APP transgene in the J20 mouse model of Alzheimer's disease.; Authors - Justin L Tosh
rat	WB	Cell Rep; Identification of dynamic RNA-binding proteins uncovers a Cpeb4-controlled regulatory cascade during pathological cell growth of cardiomyocytes.; Authors - Eva Riechert
mouse	WB	Transpl Immunol; ZBTB20 regulates cardiac allograft rejection through NFкB-mediated inflammation in mouse heart transplantation.; Authors - Liuyi Lu
human,rat	WB,IHC	Brain Res; Dysfunctional microRNA-144-3p/ZBTB20/ERK/CREB1 signalling pathway is associated with MK-801-induced schizophrenia-like abnormalities; Authors - Bo Pan