ZAK Monoclonal antibody

ZAK Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2H10E4

Cat no : 68599-1-Ig

Synonyms

AZK, HCCS 4, MLK related kinase, MLK7, mlklak, MLT, MLTK, MRK, ZAK

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Validation Data Gallery

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  WB analysis using 68599-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of rat skeletal muscle using 68599-1-Ig

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit skeletal muscle using 68599-1-Ig

  rabbit skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: U2OS cells, rat skeletal muscle tissue, rabbit skeletal muscle tissue, Saos-2 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68599-1-Ig targets ZAK in WB, ELISA applications and shows reactivity with Human, rat, rabbit samples.

• Tested Reactivity: Human, rat, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ZAK fusion protein Ag6747
• Full Name: sterile alpha motif and leucine zipper containing kinase AZK
• Calculated Molecular Weight: 91 kDa
• Observed Molecular Weight: 91 kDa
• GenBank Accession Number: BC001401
• Gene Symbol: ZAK
• Gene ID (NCBI): 51776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa, 51 kDa and 35 kDa.