WBP2NL Polyclonal antibody

WBP2NL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 22587-1-AP

Synonyms

PAWP, WBP2 N terminal like, WBP2NL

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Validation Data Gallery

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  WB analysis of human testis using 22587-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 22587-1-AP (WBP2NL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 22587-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22587-1-AP (WBP2NL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 22587-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22587-1-AP (WBP2NL Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 22587-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22587-1-AP (WBP2NL Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human testis tissue, mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 4 publications below

Product Information

The immunogen of 22587-1-AP is WBP2NL Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WBP2NL fusion protein Ag18347
• Full Name: WBP2 N-terminal like
• Calculated Molecular Weight: 309 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC022546
• Gene Symbol: WBP2NL
• Gene ID (NCBI): 164684
• RRID: AB_2879128
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun; CEP128 is involved in spermatogenesis in humans and mice.; Authors - Xueguang Zhang
human	WB	Hum Mol Genet; Homozygous mutation of PLCZ1 leads to defective human oocyte activation and infertility that is not rescued by the WW binding protein PAWP.; Authors - Jessica Escoffier
	WB,IF	Int J Mol Sci; Comprehensive Flow-Cytometric Quality Assessment of Ram Sperm Intended for Gene Banking Using Standard and Novel Fertility Biomarkers.; Authors - Jaromír Vašíček
mouse	WB	Development; PLCζ is the physiological trigger of the Ca(2+) oscillations that induce embryogenesis in mammals but offspring can be conceived in its absence.; Authors - Alaa Hachem
human	WB, IF	Mol Hum Reprod; Functional disparity between human PAWP and PLCζ in the generation of Ca2+ oscillations for oocyte activation.; Authors - Michail Nomikos
human	WB	Mol Hum Reprod; Antigen unmasking enhances visualization efficacy of the oocyte activation factor, phospholipase C zeta, in mammalian sperm.; Authors - Junaid Kashir